Cytotoxic constituents from Lobaria scrobiculata and a comparison of two bioassays for their evaluation.

نویسندگان

  • Andreas Schinkovitz
  • Aman Kaur
  • Ernst Urban
  • Martin Zehl
  • Gabriela Páchniková
  • Yuehong Wang
  • Nadine Kretschmer
  • Iva Slaninová
  • Guido F Pauli
  • Scott G Franzblau
  • Georg Krupitza
  • Rudolf Bauer
  • Brigitte Kopp
چکیده

Lichens are resilient organisms, known for their unique profiles of secondary metabolites and for exhibiting antioxidative, antibacterial, and cytotoxic effects. Analyzing the cytotoxic potential of Lobaria scrobiculata, a bioassay-guided fractionation strategy yielded seven known metabolites, with two of these compounds, 2 and 3, exhibiting cytotoxicity against HL-60 cells. In order to verify the potential impact of degradation on observed bioactivity, a purity and stability evaluation was conducted. The consistency of results obtained by the water-soluble tetrazolium salt-1 assay and trypan blue cytotoxicity assay was evaluated for selected compounds.

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عنوان ژورنال:
  • Journal of natural products

دوره 77 4  شماره 

صفحات  -

تاریخ انتشار 2014